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LVCAS9NEO

Cas9 Neomycin Lenti Particles

Synonym(s):

Lenti Particles

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Size/SKUAvailabilityPrice
1 ea
Usually ships in 3 weeks.
$419.00

About This Item

NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1 x 10 6 VP/ml virus (via p24 assay)

$419.00


Usually ships in 3 weeks.

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packaging

vial of 200 μL

Quality Segment

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

application(s)

CRISPR

selection

neomycin / G418

shipped in

dry ice

storage temp.

−70°C

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Other Notes

Click here to order gRNA plasmid and virus.
200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

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This Item
CAS9GFPVCAS9NEOCAS9RFPV
description

-

description

-

description

-

description

-

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

concentration

≥5 x 10 8 VP/ml virus (via p24 assay)

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

concentration

≥5 x 10 8 VP/ml virus (via p24 assay)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

-

packaging

vial of 200 μL

packaging

pkg of 8 x 25 μL (aliquots)

packaging

vial of 50 μL

packaging

pkg of 8 x 25 μL (aliquots)

application(s)

CRISPR

application(s)

CRISPR

application(s)

CRISPR

application(s)

CRISPR


Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Certificates of Analysis (COA)

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Questions

  1. Is there a protocol associated with the Cas9 Neomycin lentiparticles? I will be transfecting some glioblastoma cells with the virus and am looking for a starting point for protocol design.

    1 answer
    1. Titer values are lot specific and available in the Certificate of Analysis. Please navigate to the documentation section to view the sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/lvcas9neo#product-documentation

      A specific protocol for this item is not available, however this item is required for use in several Lentiviral CRISPR Pool products. Reviewing the Technical Bulletin for these products may be helpful. Please see the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/472/hwgcrisprbul.pdf

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