LYSC9000

Lys-C Protease

Cleaves at lysine residues, suitable for mass spectrometry, from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa)

• Synonym(s): Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• EC Number: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54
• Biological source: Achromobacter lyticus
• Recombinant: expressed in E. coli (206-473aa)
• Concentration: 1 mg/mL

Properties

• Product Name: Lys-c (lysyl-endopeptidase), Active, from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His Tag, solution
• biological source: Achromobacter lyticus
• recombinant: expressed in E. coli (206-473aa)
• grade: Proteomics Grade
• form: ready-to-use solution
• concentration: 1 mg/mL
• optimum pH: 8.5
• pH range: 9.0-9.5(catalytic)
• UniProt accession no.: P15636
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 100, 200

Description

General description

Recombinant His tag Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview


Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Application

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Biochem/physiol Actions

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Packaging

1mg/ml in Plastic

Preparation Note

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Other Notes

For R&D only.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable