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LYSC9001

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His-tag, powder

Synonym(s):

API, Lysyl endopeptidase, Protease I

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About This Item

EC Number:
3.4.21.50 (BRENDA, IUBMB)
NACRES:
NA.54
UNSPSC Code:
12352204
Biological source:
Achromobacter lyticus
Recombinant:
expressed in E. coli (206-473aa)

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biological source

Achromobacter lyticus

recombinant

expressed in E. coli (206-473aa)

grade

Proteomics Grade

form

lyophilized powder

optimum pH

8.5

pH range

9.0-9.5(catalytic)

UniProt accession no.

P15636

storage temp.

−20°C

Quality Level

100, 200

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This Item
LYSC9000ENDOLYSS-RO324715
Lys-c (lysyl-endopeptidase), Active from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His-tag, powder

Sigma-Aldrich

LYSC9001

Lys-c (lysyl-endopeptidase), Active

Lys-c (lysyl-endopeptidase), Active from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), His Tag, solution

Sigma-Aldrich

LYSC9000

Lys-c (lysyl-endopeptidase), Active

Endoproteinase Lys-C Sequencing Grade from Lysobacter enzymogenes

Roche

ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

Sigma-Aldrich

324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

grade

Proteomics Grade

grade

Proteomics Grade

grade

protein sequencing grade

grade

Proteomics Grade

biological source

Achromobacter lyticus

biological source

Achromobacter lyticus

biological source

bacterial (Lysobacter enzymogenes)

biological source

-

form

lyophilized powder

form

ready-to-use solution

form

lyophilized

form

lyophilized

recombinant

expressed in E. coli (206-473aa)

recombinant

expressed in E. coli (206-473aa)

recombinant

-

recombinant

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

UniProt accession no.

P15636

UniProt accession no.

P15636

UniProt accession no.

Q7M135

UniProt accession no.

-

General description

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Application

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Biochem/physiol Actions

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Packaging

1mg/ml in Plastic

Preparation Note

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.
Reconstitute in sterile water.
Store product at -20°C. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.

Other Notes

For R&D only.

pictograms

Health hazard
GHS08

signalword

Danger

Storage Class

11 - Combustible Solids

wgk

WGK 3

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCQ8663
SLCQ8663
SLCJ4572

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