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MilliporeSigma

M0275

Minimum Essential Medium Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

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Product Name

Minimum Essential Medium Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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1 of 4

This Item
M8167M2645M8028
Sigma-Aldrich

Sigma-Aldrich

M0275

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M8167

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

SAFC

M2645

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M8028

Minimum Essential Medium Eagle

form

liquid

form

liquid

form

powder

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: yes
L-glutamine: no
HEPES: no

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

components

glucose: yes
phenol red: yes
HEPES: no
Hanks’ salts (2% CO2): no
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): no
sodium pyruvate: no
stable glutamine: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel).[1][2][3][4] It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.[5]

Preparation Note

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

related product

Product No.
Description
Pricing

used together

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBL8856
RNBL4490
RNBL2068
RNBK3740
RNBJ6472

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Collagen-lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation
Vandrovcova M, et al.
Journal of Biomedical Materials Research Part A, 103(2), 525-533 (2015)
Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure
Meder R, et al.
Osteoarthritis and Cartilage, 14(9), 875-881 (2006)
Karl Norris et al.
Micromachines, 10(1) (2019-01-24)
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial
Comparison of morphology, orientation, and migration of tendon derived fibroblasts and bone marrow stromal cells on electrochemically aligned collagen constructs
Gurkan UA, et al.
Journal of Biomedical Materials Research Part A, 94(4), 1070-1079 (2010)
A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine
Drzewiecki KE, et al.
Technology, 5(04), 185-195 (2017)
View All Related Papers

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Questions

  1. If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

    1 answer

    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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