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M0275

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture, 10x

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Synonym(s):
EMEM, MEM
UNSPSC Code:
12352207
NACRES:
NA.71

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel). It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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