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M0324

Sigma-Aldrich

MEKK2, active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
MAP3K2, MEKK2B
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

192-260 nmol/min·mg

mol wt

~115 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MAP3K2(10746)

Biochem/physiol Actions

MEKK2 (also known as mitogen-activated protein kinase (MAPK) kinase kinase 2) is a major upstream activator of the JNK/MAPK cascade. MEKK2 can mediate in vitro phosphorylation of MKK4 and MKK7 which are key MAPKs that activate JNK. In addition, MEKK2 immunoprecipitates activated JUN in an IL1-dependent manner. Rheumatoid arthritis and osteoarthritis synovial tissues show high levels of MEKK2 indicating that it may be an important activator of the JNK pathway in arthritis. Endogenous MEKK2 is localized mainly in the cytosol of unstimulated cells.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Hui Li et al.
EMBO reports, 23(11), e54603-e54603 (2022-09-27)
Aberrant activation of inflammation signaling triggered by tumor necrosis factor α (TNF-α), interleukin-1 (IL-1), and interleukin-17 (IL-17) is associated with immunopathology. Here, we identify neural precursor cells expressed developmentally down-regulated gene 4-like (NEDD4L), a HECT type E3 ligase, as a
J L Blank et al.
The Journal of biological chemistry, 271(10), 5361-5368 (1996-03-08)
Mitogen-activated protein/ERK kinase kinases (MEKKs) phosphorylate and activate protein kinases which in turn phosphorylate and activate the p42/44 mitogen-activated protein kinase (MAPK), c-Jun/stress-activated protein kinases (JNKs), and p38/Hog1 kinase. We have isolated the cDNAs for two novel mammalian MEKKs (MEKK
Deepa R Hammaker et al.
Journal of immunology (Baltimore, Md. : 1950), 172(3), 1612-1618 (2004-01-22)
The mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK) is a critical regulator of collagenase-1 production in rheumatoid arthritis (RA). The MAPKs are regulated by upstream kinases, including MAPK kinases (MAPKKs) and MAPK kinase kinases (MAP3Ks). The present study was
Mingyue Wen et al.
Nature communications, 6, 7167-7167 (2015-05-20)
NDR/LATS kinase family is highly conserved from yeast to human. It remains unknown whether the members of this family function in innate immune responses. Here we demonstrate that Stk38 negatively regulates TLR9-mediated immune responses in macrophages. Stk38 constitutively associates with

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