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M0393

Sigma-Aldrich

Medium 199

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
M199 Medium, TCM 199
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
L-glutamine: yes
HEPES: no
NaHCO3: no
Hanks’ salts (2% CO2): yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Medium 199 was originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Articles

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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