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M041130

Sigma-Aldrich

6-Fluorescein Phosphoramidite

configured for ABI

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Synonym(s):
6-FAM-phosphoramidite
Empirical Formula (Hill Notation):
C46H58N3O10P
CAS Number:
Molecular Weight:
843.94
MDL number:
PubChem Substance ID:
NACRES:
NA.21

type

for DNA synthesis

Quality Level

product line

Proligo Reagents

Assay

≥97% (31P-NMR)
≥97.0% (reversed phase HPLC)

color

solid white to off-white

solubility

acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)

λ

conforms (UV/VIS Identity)

suitability

conforms to structure for H-NMR

compatibility

configured for ABI

storage temp.

−20°C

SMILES string

CC(C)N(C(C)C)P(OCCCCCCNC(=O)c1ccc2C(=O)OC3(c4ccc(OC(=O)C(C)(C)C)cc4Oc5cc(OC(=O)C(C)(C)C)ccc35)c2c1)OCCC#N

InChI

1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-34-37(26-31)46(59-41(34)51)35-20-17-32(56-42(52)44(5,6)7)27-38(35)58-39-28-33(18-21-36(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)

InChI key

MGPYJVWEJNTXLC-UHFFFAOYSA-N

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This Item
M041180M043130M043180
assay

≥97% (31P-NMR), ≥97.0% (reversed phase HPLC)

assay

≥97% (31P-NMR), ≥97.0% (reversed phase HPLC)

assay

≥97% (31P-NMR), ≥97.0% (reversed phase HPLC)

assay

≥97% (31P-NMR), ≥97.0% (reversed phase HPLC)

color

solid white to off-white

color

solid white to off-white

color

white to off-white, powder

color

white to off-white, powder

solubility

acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)

solubility

acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)

solubility

-

solubility

-

suitability

conforms to structure for H-NMR

suitability

conforms to structure for H-NMR

suitability

conforms to structure for H-NMR

suitability

conforms to structure for H-NMR

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Phosphoramidite technology is ideally suited for the modification ofsynthetic oligonucleotides with covalently attached reporter moieties,linkers, spacers, or haptens. Sigma-Aldrich provides popular reporters andlinkers ready to use for DNA/RNA synthesis machines such as β-cyanoethylphosphoramidites. 6-Fluorescein Phosphoramidite is one such label whose absorbance maxima is 496 nm and emission maxima is 520 nm.Key Features of 6-Fluorescein Phosphoramidite:

  • 6-Fluorescein Phosphoramidite consists of one isomer derived from6-carboxy fluorescein
  • Couples to the 5′-end of the oligonucleotide using standard synthesisprotocols
  • Protecting groups on the fluorescein moiety are removed under standardconditions of cleavage and deprotection with concentrated ammonia
  • A detritylation step is not required since the fluorescein phosphoramiditedoes not contain a DMT-group
  • The labeled oligonucleotide is ready for use in most applications after theevaporation of ammonia. Standard procedures can be used if additionalpurification is required.
  • Lot-to-lot consistent purity and performance
  • Manufactured under a certified ISO 9001 quality system6-Fluorescein Phosphoramidite is configured for ABI Synthesizers.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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