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M0446

Sigma-Aldrich

StableCell™ MEM

With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Minimum Essential Medium Eagle, MEM
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

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I2911R2405D0697
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M0446

StableCell™ MEM

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I2911

StableCell™ IMDM

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R2405

StableCell™ RPMI-1640

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D0697

StableCell™ DMEM/F12

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

Endotoxin, tested

impurities

endotoxin, tested

impurities

Endotoxin, tested

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

components

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

components

sodium pyruvate: no
NaHCO3: yes
phenol red: yes
stable glutamine: yes
HEPES: no

components

glucose: high
sodium pyruvate: yes
stable glutamine: yes
NaHCO3: yes
phenol red: yes
HEPES: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Application

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Legal Information

StableCell is a trademark of Sigma-Aldrich Co. LLC

also commonly purchased with this product

Product No.
Description
Pricing

recommended

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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