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M0450

Minimum Essential Medium Eagle

Alpha Modification, with nucleosides, ʟ-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EMEM, MEM

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500 mL
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$71.40

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

$71.40


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Product Name

Minimum Essential Medium Eagle, Alpha Modification, With ribonucleosides, L-glutamine, deoxyribonucleosides and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. See formulation for details. Often used in cytogenetic analysis when supplemented with FBS.

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This Item
M0200M8042M2279
form

liquid

form

liquid

form

liquid

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

components

phenol red: 0.011 g/L
glucose: 1.0 g/L
L-glutamine: 0.292 g/L
NaHCO3: 2.2 g/L

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Optimization of chemically defined cell culture media-replacing fetal bovine serum in mammalian in vitro methods
Van der VJ, et al.
Toxicology in vitro, 24(4), 1053-1063 (2010)



Global Trade Item Number

SKUGTIN
M0450-500ML04061837847288

Questions

  1. What is the concentration of L-glutamine present in this medium?

    1 answer
    1. As mentioned in the 'PROPERTIES' section, the concentration of L-glutamine in this product is 0.292 g/L. Please see the link below to review the full formulation:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/593/733/m0450for.pdf

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