MilliporeSigma
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M0518

Sigma-Aldrich

Minimum Essential Medium Eagle

Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, suitable for cell culture

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Synonym(s):
JMEM, Joklik Modified MEM, MEM
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
M8167M0644M0268
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
phenol red: yes

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: yes
L-glutamine: no
HEPES: no

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

400

Quality Level

500

Quality Level

500

Application

Recommended for suspension cultures.

Quantity

Formulated to contain 11.0 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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