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M0518

Minimum Essential Medium Eagle

Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, suitable for cell culture

Synonym(s):

JMEM, Joklik Modified MEM, MEM

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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This Item
M2645M8028M8167
Sigma-Aldrich

Sigma-Aldrich

M0518

Minimum Essential Medium Eagle

Minimum Essential Medium Eagle HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

SAFC

M2645

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M8028

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M8167

Minimum Essential Medium Eagle

form

powder

form

powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
phenol red: yes

components

HEPES: 25 mM
phenol red: yes
L-glutamine: yes
NaHCO3: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes

components

glucose: yes
phenol red: yes
HEPES: no
Hanks’ salts (2% CO2): no
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): no
sodium pyruvate: no
stable glutamine: no

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: yes
L-glutamine: no
HEPES: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

-

Quality Level

400

Quality Level

400

Application

Recommended for suspension cultures.

Preparation Note

Formulated to contain 11.0 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.

related product

Product No.
Description
Pricing

used together

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000513451
0000513453
0000513451
0000513453
25D440

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Nirmal Verma et al.
Scientific reports, 11(1), 20926-20926 (2021-10-24)
Population-based studies identified an association between a prior pregnancy complicated by gestational diabetes mellitus (GDM) and cardiac hypertrophy and dysfunction later in life. It is however unclear whether GDM initiates this phenotype and what are the underlying mechanisms. We addressed
Christopher A Drummond et al.
Physiological genomics, 48(3), 220-229 (2015-12-25)
Chronic kidney disease (CKD) is accompanied by cardiac fibrosis, hypertrophy, and dysfunction, which are commonly referred to as uremic cardiomyopathy. Our previous studies found that Na/K-ATPase ligands or 5/6th partial nephrectomy (PNx) induces cardiac fibrosis in rats and mice. The
Winston T Stauffer et al.
International journal of molecular sciences, 21(4) (2020-02-23)
Activating transcription factor-6 α (ATF6) is one of the three main sensors and effectors of the endoplasmic reticulum (ER) stress response and, as such, it is critical for protecting the heart and other tissues from a variety of environmental insults
Francesca Rivello et al.
Science advances, 6(40) (2020-10-02)
Despite their important role in metastatic disease, no general method to detect circulating stromal cells (CStCs) exists. Here, we present the Metabolic Assay-Chip (MA-Chip) as a label-free, droplet-based microfluidic approach allowing single-cell extracellular pH measurement for the detection and isolation
Giulia Elisa Cambi et al.
Molecular bioSystems, 8(9), 2351-2359 (2012-06-28)
Although angiotensin (Ang)II-induced Janus-activated kinase (JAK)2 phosphorylation was reported to be enhanced in failing human cardiomyocytes, the downstream balance between cardio-protective (signal transducer and activator of transcription-STAT3) and the pro-inflammatory (STAT2 and STAT5) response remains unexplored. Therefore STATs phosphorylation and
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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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