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M0518

Sigma-Aldrich

Minimum Essential Medium Eagle

Joklik Modification, with L-glutamine, without calcium chloride and sodium bicarbonate, suitable for cell culture

Synonym(s):
JMEM, Joklik Modified MEM, MEM
NACRES:
NA.75

form

powder

application(s)

cell culture | mammalian: suitable

components

HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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Related Categories

Application

Recommended for suspension cultures.

Quantity

Formulated to contain 11.0 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Giulia Elisa Cambi et al.
Molecular bioSystems, 8(9), 2351-2359 (2012-06-28)
Although angiotensin (Ang)II-induced Janus-activated kinase (JAK)2 phosphorylation was reported to be enhanced in failing human cardiomyocytes, the downstream balance between cardio-protective (signal transducer and activator of transcription-STAT3) and the pro-inflammatory (STAT2 and STAT5) response remains unexplored. Therefore STATs phosphorylation and...
Francesca Rivello et al.
Science advances, 6(40) (2020-10-02)
Despite their important role in metastatic disease, no general method to detect circulating stromal cells (CStCs) exists. Here, we present the Metabolic Assay-Chip (MA-Chip) as a label-free, droplet-based microfluidic approach allowing single-cell extracellular pH measurement for the detection and isolation...
Winston T Stauffer et al.
International journal of molecular sciences, 21(4) (2020-02-23)
Activating transcription factor-6 α (ATF6) is one of the three main sensors and effectors of the endoplasmic reticulum (ER) stress response and, as such, it is critical for protecting the heart and other tissues from a variety of environmental insults...
Yipeng Yang et al.
Experimental biology and medicine (Maywood, N.J.), 242(3), 286-296 (2016-10-07)
Hypothyroidism is a common condition of hormone deficiency, and oral administration of thyroid hormones is currently the only available treatment option. However, there are some disadvantages with this treatment modality including compliance challenges to patients. Therefore, a physiologically based alternative...
Kevin T James et al.
Scientific reports, 6, 36826-36826 (2016-11-09)
Viruses are extensively studied as pathogens and exploited as molecular tools and therapeutic agents. Existing methods to purify viruses such as gradient ultracentrifugation or chromatography have limitations, for example demand for technical expertise or specialized equipment, high time consumption, and...

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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