All Photos(1)



Phosphate-ON Phosphoramidite

configured for MerMade

Sign Into View Organizational & Contract Pricing

Select a Size

Phosphate-ON amidite
Empirical Formula (Hill Notation):
Molecular Weight:
MDL number:
PubChem Substance ID:


for DNA synthesis

product line

Proligo Reagents


≥98% (31P-NMR)
≥98.0% (reversed phase HPLC)


configured for MerMade

storage temp.


SMILES string




InChI key


Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Phosphate-ON phosphoramidite is a chemical phosphorylation reagent that can be employed to introduce a phosphate group at the 5′ terminus of an oligonucleotide. Chemical phosphorylation is a cost-effective alternative to enzymatic phosphorylation methods, allowing the introduction of terminal phosphate groups in high yields, at any desired scale. We offer Phosphate-ON phosphoramidite in powder form, enabling the dissolution of the reagent in acetonitrile to be easily monitored.


The application of Phosphate-ON Phosphoramidite is fully compatible with standard cleavage and deprotection procedures at the end of oligonucleotide synthesis. Phosphate-ON phosphoramidite comprises a thymidine nucleoside, which is removed from the oligonucleotide during the cleavage and deprotection process.

Other Notes

The key features of Phosphate-ON Phosphoramidite are as follows:
  • Powder format enables dissolution to be easily monitored and facilitatesproduct handling
  • Fully compatible with standard phosphoramidite reagents and synthesisconditions
  • Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotidecan be cleaved from the support and deprotected in concentratedammonia at 55 °C in only 2 hours
  • Comprises a DMT-group for trityl monitoring
  • Dissolves easily in acetonitrile to a 0.1 M solution
  • High coupling efficiency of ≥98.0% under standard DNA phosphoramiditecoupling conditions
  • Cleavage of both phosphate protective groups through a β-eliminationprocess

Legal Information

This product is covered under US Patent Number 7,276,598

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service