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MilliporeSigma

M0643

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

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Product Name

Minimum Essential Medium Eagle, With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

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1 of 4

This Item
M7278M3024M4526
Sigma-Aldrich

Sigma-Aldrich

M0643

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M7278

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M3024

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M4526

Minimum Essential Medium Eagle

form

powder

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

components

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

500

Quality Level

400

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63[1], dispersed ventricular cells[2], and endometrial adenocarcinoma Ishikawa cells.[3]

General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids.[4] It is widely used as a media formulation for many cell culture studies.[5] It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.[4]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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Description
Pricing

used together

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000500472
0000500472
25D291
25D291
0000446770

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Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Sarah M Davis et al.
Molecular therapy. Nucleic acids, 21, 266-277 (2020-07-02)
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo. However, chemical modifications variably impact target silencing for different siRNA sequences
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Atomic layer deposited TiO2 films on an equiatomic NiTi shape memory alloy for biomedical applications
Abbas A, et al.
Journal of alloys and compounds, 161282-161282 (2021)
Is it time to reinvent basic cell culture medium?
Turney J McKee et al.
American journal of physiology. Cell physiology, 312(5), C624-C626 (2017-02-24)
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Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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