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M0643

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
MEM
NACRES:
NA.75

Quality Level

form

powder

application(s)

cell culture | mammalian: suitable

components

NaHCO3: no
Earle’s salts (5% CO2): yes
L-glutamine: yes
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Sarah M Davis et al.
Molecular therapy. Nucleic acids, 21, 266-277 (2020-07-02)
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo. However, chemical modifications variably impact target silencing for different siRNA sequences...
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Erika Nahomy Marino-Marmolejo et al.
BMC microbiology, 16(1), 290-290 (2016-12-14)
Pharmaceutical industry demands innovation for developing new molecules to improve effectiveness and safety of therapeutic medicines. Preclinical assays are the first tests performed to evaluate new therapeutic molecules using animal models. Currently, there are several models for evaluation of treatments...
Vidhya Yarahalli Jayaram et al.
Drug and chemical toxicology, 43(6), 637-644 (2018-11-15)
This study was designed to investigate possible interference of Xenobiotics with SUMOylation in eukaryotic cells. To begin with, we docked 71 chemical structures from PubChem with human SUMO1 and UBC9 protein structures using Auto Dock 4.2 and Hex 6.3 and...
Mihaela Balas et al.
Nanomaterials (Basel, Switzerland), 8(7) (2018-07-07)
Magnetic nanoparticles offer multiple utilization possibilities in biomedicine. In this context, the interaction with cellular structures and their biological effects need to be understood and controlled for clinical safety. New magnetic nanoparticles containing metallic/carbidic iron and elemental silicon phases were...

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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