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M0644

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine, ribonucleosides and deoxyribonucleosides, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

MEM

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10 L

Available to ship TODAYfromMILWAUKEE

$102.00
10 x 1 L

Available to ship TODAYfromMILWAUKEE

$134.00

About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

$102.00

Available to ship TODAYDetails

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium (MEM) is a synthetic cell culture media developed by Harry Eagle. This medium has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to this formulation that incorporates either Hanks′ or Earle′s salts has broadened the usage of this medium. The α modification of MEM with Earle′s balanced salts also known as αMEM, contains non-essential amino acids, sodium pyruvate, and additional vitamins.

Application

Minimum essential medium Eagle has been used to:

  • maintain natural killer (NK) cell lines[1]
  • culture Leishmania donovani strains[2]
  • culture epidermal neural crest stem cells (EPI-NCSCs) from the bulge of rat hair follicles[3]

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

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M0644

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M5650

Minimum Essential Medium Eagle

Sigma-Aldrich

Sigma-Aldrich

M4526

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Sigma-Aldrich

Sigma-Aldrich

M2279

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form

powder

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

400

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000519880
0000519878
0000519878
0000519880
25G270

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Hannah Finke et al.
Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie, 79(5), 328-336 (2018-07-18)
Miniscrews are an important choice for orthodontic anchorage. Yet reports on failures do exist, and attempts have been made to elucidate the causes. Clinical outcomes may be compromised not only by the mechanical implications of miniscrew design and the location
C C Lachaud et al.
Cell death & disease, 5, e1304-e1304 (2014-06-27)
Recent studies suggested that the post-natal mesothelium retain differentiative potential of the embryonic mesothelium, which generates fibroblasts and vascular smooth muscle cells (VSMCs), in developing coelomic organs via epithelial-to-mesenchymal transition (EMT). Whether adult mesothelial cells (MCs) are able to give
Kyoko Fujisawa et al.
PLoS neglected tropical diseases, 15(7), e0009552-e0009552 (2021-07-20)
Visceral leishmaniasis (VL) is a zoonotic protozoal vector-borne disease that is a major public health challenge. In Argentina, canine (CVL) and human visceral leishmaniasis (HVL) have recently emerged. There is a lack of standardised diagnostic tests for CVL, which hinders
View All Related Papers

Global Trade Item Number

SKUGTIN
M0644-10X1L04061833993798
M0644-10L04061833993781
M0644-BG04061838414595
M0644-1L04061837459528

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