Ethyl methanesulfonate


Ethyl mesylate, Methanesulfonic acid ethyl ester
Linear Formula:
CAS Number:
Molecular Weight:
Beilstein/REAXYS Number:
EC Number:
MDL number:
PubChem Substance ID:



Quality Level

refractive index

n20/D 1.418 (lit.)


85-86 °C/10 mmHg (lit.)


1.206 g/mL at 20 °C

SMILES string




InChI key


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Ethyl methanesulfonate had been used for inducing mutation in mouse embryonic fibroblasts, yeast human embryonic kidney cell lines and human lymphocytes.


1, 5, 10, 25, 100 g in glass bottle

Biochem/physiol Actions

Ethyl methanesulfonate is a DNA ethylating agent, mutagenic to plants and animals and carcinogenic in mammals. It has been used as a model alkylating agent in studies of DNA repair processes. EMS induces base substitutions of guanine-cytosine (G/C) to adenine-thymine (A/T). EMS also generates point mutations and single nucleotide polymorphisms in genomes. EMS is potential chemical mutagen used for inducing mutation in rice, wheat and Arabidopsis thaliana.


Exclamation markHealth hazard

Signal Word


Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


UN 3082 9 / PGIII

WGK Germany


Flash Point(F)

212.0 °F

Flash Point(C)

100 °C

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. I have heard that I need to use sodium thiosulfate with Product M0880, Ethyl methanesulfonate (EMS), but I’m not sure how. Can you tell me?

    EMS is a mutagen. After treating cells with EMS, the mutagenesis reaction may be stopped by adding the appropriate volume of a 5% sodium thiosulfate solution.The use of a 10% sodium thiosulfate solution with 1% TWEEN 20 has been used to clean surfaces and items such as pipettes that have been contaminated with EMS in mineral oil.

  4. Is Product M0880, Ethyl methanesulfonate (EMS), sterile?

    It is not necessarily sterile. For those applications that require a sterile EMS solution, the EMS should be added to the appropriate buffer or medium. The buffer or medium should then be sterile-filtered.

  5. I received a liquid. What is Product M0880, Ethyl methanesulfonate (EMS), dissolved in?

    It’s actually not dissolved in anything. Pure EMS is itself a liquid at room temperature; that is, the melting point is below room temperature.

  6. How does Product M0880, Ethyl methanesulfonate (EMS), compare to Product 64292, which is the same chemical?

    Product No. 64292 is tested for purity by a titration assay. Although M0880 is likely to be of similar quality, there is no purity assay performed on it.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

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Genotoxicity Effect of Ethyl Methanesulfonate on Root Tip Cells of Chilli (Capsicum annuum L.)
Devi AS and Mullainathan L
World Journal of Agricultural Sciences, 7(4), 368-374 (2011)
Scanning ethyl methanesulphonate effects on the whole genome of Lotus japonicus using second generation sequencing analysis
Mohd-Yusoff NF, et al.
G3 (Bethesda, Md.), g3-114 (2015)
Novel wine yeast with ARO4 and TYR1 mutations that overproduce `floral?aroma compounds 2-phenylethanol and 2-phenylethyl acetate
Cordente AG, et al.
Applied Microbiology and Biotechnology, 1-12 (2018)
Kade M Power et al.
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A Rapid and Precise Mutation-Activated Fluorescence Reporter for Analyzing Acute Mutagenesis Frequency
Birnbaum MD, et al.
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