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M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

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Synonym(s):
MgCl2, dichloromangesium, magnesium chloride
Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

technique(s)

DNA sequencing: suitable

impurities

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for electrophoresis
suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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General description

Magnesium chloride is a common ionic halide that readily dissolves in water. It plays various crucial biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid composition. Additionally, magnesium chloride serves as an essential co-factor in numerous enzymes, including DNAse, specific restriction enzymes, and Ribonuclease H. It is widely utilized to provide magnesium ions in diverse molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It has been used -
  • to prepare buffer to lyse cells for immunoprecipitation
  • as a supplement in Krebs-Henseleit buffer in chronic high-fat diet studies of aged mice.
  • in embedding 3-dimensional mtgase hydrogels in invitro studies of osteocyte differentitaion and interconnected cellular networks.
  • It has been used to protect the liver from oxidative damage.
  • to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Features and Benefits

  • Available as a Ready-to-use solution which reduces the need for preparation time.
  • Heavy metals (as Pb): ≤5 ppm
  • Free from DNase, RNase, NICKase and protease

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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