M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• Synonym(s): Moloney Murine Leukemia Virus Reverse Transcriptase
• CAS Number: 9068-38-6
• MDL number: MFCD00283754
• NACRES: NA.55

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• usage: sufficient for 200 reactions; sufficient for 250 reactions
• feature: dNTPs included: no; hotstart: no
• concentration: 200 units/μL
• technique(s): RT-PCR: suitable
• color: colorless
• input: purified RNA
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Application

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis
• for use in a 2-step RT-PCR assay
• in quantitative realtime-polymerase chain reaction (RT-qPCR)
• in reverse transcription

Packaging

1000, 40000, 200000 units

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Features and Benefits

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

Unit Definition

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves