M1421

Sigma-Aldrich

IgA, Kappa from murine myeloma

clone TEPC 15, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Mouse IgA-κ
MDL number:
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

clone

TEPC 15, monoclonal

assay

≥90% (non-reduced, SDS-PAGE)

form

buffered aqueous solution

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

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General description

IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces
IgA, κ from murine myeloma is produced by TEPC 15 tumor line that is pristane-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. The antibody is specific for κ chain of mouse IgA. The purified immunoglobulin preparation is non-reactive with anti-mouse IgM, IgG1, IgG2a, IgG2b, and IgG3.
The TEPC 15 tumor line that produces mouse IgA,k is pristane-induced plasmacytoma originated and carried intraperitoneally in BALB/c mice. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. IgA molecules in the serum are mostly dimeric. IgA is found in saliva, tracheobronchial secretions, genitourinary secretions, milk and colostrum.

Specificity

Antigen Specificity: phosphoryl choline

Application

Anti-Mouse IgA, κ antibody was used as secondary antibody in ELISA and immunoflourescence.
IgA, Kappa from murine myeloma has been used in:
  • immunofluorescence assay
  • enzyme-linked immunosorbent assay (ELISA)
  • invasion assays
  • western blotting

Biochem/physiol Actions

Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0, containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Lindsey R Brown et al.
Frontiers in cellular and infection microbiology, 7, 233-233 (2017-06-24)
Bacteria growing within biofilms are protected from antibiotics and the immune system. Within these structures, horizontal transfer of genes encoding virulence factors, and promoting antibiotic resistance occurs, making biofilms an extremely important aspect of pneumococcal colonization and persistence. Identifying environmental...
The Laboratory Rat (1998)
Carlos J Sanchez et al.
PloS one, 6(12), e28738-e28738 (2011-12-17)
It is unclear whether Streptococcus pneumoniae in biofilms are virulent and contribute to development of invasive pneumococcal disease (IPD). Using electron microscopy we confirmed the development of mature pneumococcal biofilms in a continuous-flow-through line model and determined that biofilm formation...
AdcAII of Streptococcus pneumoniae affects pneumococcal invasiveness
Brown LR, et al.
PLoS ONE, 11(1), e0146785-e0146785 (2016)
Yunhao Tan et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 21212-21217 (2011-12-14)
Effectors delivered into host cells by the Legionella pneumophila Dot/Icm type IV transporter are essential for the biogenesis of the specialized vacuole that permits its intracellular growth. The biochemical function of most of these effectors is unknown, making it difficult...

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