M1533

N,N′-Methylenebisacrylamide solution

suitable for electrophoresis, 2% in H₂O

• Synonym(s): BIS
• Linear Formula: (CH₂=CHCONH)₂CH₂
• CAS Number: 110-26-9
• Molecular Weight: 154.17
• UNSPSC Code: 41105319
• NACRES: NB.22
• PubChem Substance ID: 24896663
• MDL number: MFCD00008625
• Beilstein/REAXYS Number: 1706297

Properties

• sterility: 0.2 μm filtered
• Quality Segment: 200
• form: liquid
• concentration: 2% in H₂O
• technique(s): electrophoresis: suitable
• storage temp.: 2-8°C
• SMILES string: C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
• InChI key: ZIUHHBKFKCYYJD-UHFFFAOYSA-N

Description

General description

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.

Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication.[1][2] It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.[3]

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Preparation Note

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H340,H350,H373
• pcodes: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B - Muta. 1B - STOT RE 2 Oral
• target_organs: Peripheral nervous system
• Storage Class: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)