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M1567

Malic Dehydrogenase from porcine heart

≥600 units/mg protein (biuret), ammonium sulfate suspension

Synonym(s):

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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5000 units
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$115.00
10000 units
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$200.00
25000 units
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$398.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-622-5
MDL number:
MFCD00131567
EC Number:
1.1.1.37 (BRENDA, IUBMB)
Specific activity:
≥600 units/mg protein (biuret)

$115.00

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form

ammonium sulfate suspension

Quality Level

200

specific activity

≥600 units/mg protein (biuret)

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

storage temp.

2-8°C

General description

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity. [1]
Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.
Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.

Application

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. [2] It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.
Malic Dehydrogenase from porcine heart has been used:
  • in qualitative protein binding measurements
  • to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
  • to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Biochem/physiol Actions

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH+. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

Physical form

Suspension in 2.8 M (NH4)2SO4 solution, pH 6.0

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25°C.

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Malic Dehydrogenase from porcine heart ≥600 units/mg protein (biuret), ammonium sulfate suspension

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M1567

Malic Dehydrogenase from porcine heart

Malic Dehydrogenase from porcine heart buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Sigma-Aldrich

M2634

Malic Dehydrogenase from porcine heart

Malic Dehydrogenase from bovine heart ammonium sulfate suspension, 2000-4000 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

M9004

Malic Dehydrogenase from bovine heart

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)

Sigma-Aldrich

M5257

Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver

specific activity

≥600 units/mg protein (biuret)

specific activity

600-1000 units/mg protein (biuret)

specific activity

2000-4000 units/mg protein (modified Warburg-Christian)

specific activity

10-30 units/mg protein (modified Warburg-Christian)

form

ammonium sulfate suspension

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

foreign activity

-

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000497754
0000497755
0000497754
0000270147
0000441927

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Protocols

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Malate dehydrogenases-structure and function
Minarik P, et al.
General Physiology and Biophysics, 21(3), 257-266 (2002)
Sensing NADH conformation using phasor analysis on fluorescence spectra
Palo D, et al.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 186(3), 105-111 (2017)
Yassir M Al-Mulla Hummadi et al.
Experimental parasitology, 112(2), 85-91 (2005-11-09)
S(2) complex has been reported to have a direct antileishmanial effect. The possibility that the direct antileishmanial effect may be due to inhibition of key enzymes involved in glucose metabolism and/ or enzymes associated with virulence was investigated. Cell pellets
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Global Trade Item Number

SKUGTIN
M1567-10KU04061834040910
M1567-25KU04061832730868
M1567-5KU04061834040927

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