M1570

Anti-Myosin (Skeletal, Fast) antibody, Mouse monoclonal

clone MY-32, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse
• MDL number: MFCD00145920
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MY-32, monoclonal
• form: buffered aqueous solution
• species reactivity: rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
• packaging: antibody small pack of 25 μL
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue; microarray: suitable; western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle
• isotype: IgG1
• UniProt accession no.: P12882
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... MYH1(4619) , MYH2(4620) ; mouse ... Myh1(17879) , Myh2(17882) ; rat ... Myh1(287408) , Myh2(691644)

Description

General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Immunogen

rabbit muscle myosin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

Packaging

25, 100, 200 μL

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1