M220

Monoclonal Anti-Nicotinic Acetylcholine Receptor (α₇ Subunit) antibody produced in mouse

~5 mg/mL, clone mAb 306, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-CHRNA7-2, Anti-NACHRA7
• MDL number: MFCD00677279

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mAb 306, monoclonal
• form: buffered aqueous solution
• species reactivity: rat (denatured), human (denatured), chicken
• concentration: ~5 mg/mL
• technique(s): immunohistochemistry (frozen sections): 1:3,000
• isotype: IgG1
• UniProt accession no.: P36544
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CHRNA7(1139) ; rat ... Chrna7(25302)

Description

General description

Nicotinic acetylcholine receptors (nAChRs) function as ligand-gated channels that modulate endoplasmic reticulum stress and neurotransmitter release. These receptors also control synaptic signaling in ganglia and muscles. The α7 form of nAChR is expressed in mitochondria and regulates Ca²⁺ transport and the release of cytochrome c. When expressed in non-excitable cells, α7 nAChR mediates cell growth and survival
Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is specific for native and denatured α7 subunits in chick, as well as for the denatured α7 subunits in rats and humans. The antibody does not bind well to the native form of the mammalian α7 subunit. The product can be used in chick, rat and human tissues.

Immunogen

native and denatured α₇ subunit (amino acids 380-400, binds α-bungarotoxin) of the nicotinic acetylcholine receptor from chicken and the denatured α₇ subunit from rat.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal anti-nicotinic acetylcholine receptor ((α7) subunit) antibody is suitable for use in immunocytochemistry (at 1:3000-1:50 dilutions using paraformaldehyde fixed, cultured rat chromaffin cells) , and immunohistochemistry (at 1:3,000 dilutions using frozen sections).

Packaging

100 μL

Physical form

Solution in 20 mM Na₃PO₄, pH 7.2, containing 150 mM NaCl and 0.05% sodium azide.

Legal Information

Sold with permission of the Salk Institute

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves