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M2405

Sigma-Aldrich

4-Methylumbelliferyl β-D-lactopyranoside

β-galactosidase substrate

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Synonym(s):
4-Methylumbelliferyl β-D-lactoside
Empirical Formula (Hill Notation):
C22H28O13
CAS Number:
Molecular Weight:
500.45
Beilstein:
8094402
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

Assay

≥98.00% (TLC)

form

powder

solubility

water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 365 nm; λem 445 nm (after enzymatic degradation)

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O)[C@H]4O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C22H28O13/c1-8-4-14(25)32-11-5-9(2-3-10(8)11)31-21-19(30)17(28)20(13(7-24)34-21)35-22-18(29)16(27)15(26)12(6-23)33-22/h2-5,12-13,15-24,26-30H,6-7H2,1H3/t12-,13-,15+,16+,17-,18-,19-,20-,21-,22+/m1/s1

InChI key

PRTGXBPFDYMIJH-KSFLKEQHSA-N

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This Item
M0905M9766M6018
form

powder

form

powder

form

powder

form

powder

solubility

water: 9.80-10.20 mg/mL, clear, colorless to faintly yellow

solubility

pyridine: 10 mg/mL, clear, colorless to faintly yellow

solubility

pyridine: 50 mg/mL, clear, colorless to faintly yellow

solubility

water: 19.60-20.40 mg/mL, clear, colorless to faintly yellow

fluorescence

λex 365 nm; λem 445 nm (after enzymatic degradation)

fluorescence

-

fluorescence

-

fluorescence

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

200

Application

4-Methylumbelliferyl β-D-lactopyranoside has been used as a chromophoric substrate for cellobiohydrolase I (CBHI) in T. reesei. It has also been used as a substrate for cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase from fermented samples in enzymatic assay.

Biochem/physiol Actions

4-Methylumbelliferyl β-D-lactopyranoside is a fluorescence compound hydrolyzed by the enzyme β-glucosidase (βG) and CBH1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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M J Bailey et al.
Applied microbiology and biotechnology, 62(2-3), 156-162 (2003-04-08)
A low-foaming hydrophobin II deletant of the Trichoderma reesei strain Rut-C30 was used for production of cellulases by continuous cultivation on lactose medium in a laboratory fermenter. The control paradigm of the addition of new medium to the continuous process
Ana Cristina Rodrigues et al.
Bioresource technology, 156, 163-169 (2014-02-08)
The potential of enzymes recycling after hydrolysis and fermentation of wheat straw under a variety of conditions was investigated, monitoring the activity of the enzymes in the solid and liquid fractions, using low molecular weight substrates. A significant amount of
Yaron Shav-Tal et al.
FEBS letters, 531(2), 109-114 (2002-11-06)
Proteins are often referred to in accordance with the activity with which they were first associated or the organelle in which they were initially identified. However, a variety of nuclear factors act in multiple molecular reactions occurring simultaneously within the
Ana Cristina Rodrigues et al.
Enzyme and microbial technology, 79-80, 70-77 (2015-09-01)
The hydrolysis/fermentation of wheat straw and the adsorption/desorption/deactivation of cellulases were studied using Cellic(®) CTec2 (Cellic) and Celluclast mixed with Novozyme 188. The distribution of enzymes - cellobiohydrolase I (Cel7A), endoglucanase I (Cel7B) and β-glucosidase - of the two formulations
Tassula Proikas-Cezanne et al.
FEBS letters, 580(22), 5241-5246 (2006-09-12)
Rab14 localizes to the Golgi/TGN and to early endosomes, but its biological function remains unclear. By structural modeling, we identified Rab14-specific residues and established a close relationship between the Rab2/Rab4/Rab14, Rab11/25 and Rab39 sub-groups within the Rab protein family. By

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