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M2634

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

Synonym(s):
Malate Dehydrogenase, L-Malate: NAD+ oxidoreductase, MDH
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

buffered aqueous glycerol solution

Quality Level

specific activity

600-1000 units/mg protein (biuret)

cation traces

NH4+: ≤10 μg/mg protein

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

shipped in

wet ice

storage temp.

2-8°C

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General description

Mitochondrial Isozyme
Malic dehydrogenase is a mitochondrial isozyme and an important catalyst in the tricarboxylic acid cycle.

Application

Malic dehydrogenase has been used in a study to assess the comparative molluscicidal action of Ginko biloba extract on snail hosts. It has also been used in a study to investigate the effect of early feed restriction on metabolic programming and compensatory growth in broiler chickens.

Biochem/physiol Actions

Eukaryotic cells contain two different isozymes of malate dehydrogenase: mitochondrial (m-MDH) and soluble or cytoplasmic (s-MDH). This product consists of the mitochondrial form.
The enzyme catalyzes the following reaction:Oxaloacetate + β-NADH → L-Malate + β-NAD
Malic Dehydrogenase can be reversibly inactivated by treatment with pyridoxal 5′-phosphate at 25 °C.

Unit Definition

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol containing 0.05 M potassium phosphate buffer, pH 7.5

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Comparative molluscicidal action of extract of Ginko biloba sarcotesta, arecoline and niclosamide on snail hosts of Schistosoma japonicum
Chen, S., et al.
Pesticide Biochemistry and Physiology, 89, 237-241 (2007)
S S Chen et al.
The Biochemical journal, 151(2), 297-303 (1975-11-01)
1. Pig heart mitochondrial malate dehydrogenase incubated with pyridoxal 5'-phosphate at pH 8.0 and 25 degrees C gradually loses activity. Such inactivation can be largely reversed by dialysis or by addition of L-lysine or L-cysteine, and can be made permanent
X A Zhan et al.
Poultry science, 86(4), 654-660 (2007-03-21)
The effect of early feed restriction on metabolic programming and compensatory growth was studied in broiler chickens. A total of 480 female 1-d-old broiler birds (Aconred) were randomly allocated to ad libitum and feed-restricted groups, each of which was replicated
Xiaoting Ye et al.
Microbial cell factories, 11, 120-120 (2012-09-07)
The integration of biotechnology into chemical manufacturing has been recognized as a key technology to build a sustainable society. However, the practical applications of biocatalytic chemical conversions are often restricted due to their complexities involving the unpredictability of product yield
Riyadh Muhaidat et al.
Annals of botany, 111(4), 577-589 (2013-02-08)
C4 eudicot species are classified into biochemical sub-types of C4 photosynthesis based on the principal decarboxylating enzyme. Two sub-types are known, NADP-malic enzyme (ME) and NAD-ME; however, evidence for the occurrence or involvement of the third sub-type (phosphoenolpyruvate carboxykinase; PEP-CK)

Protocols

Enzymatic Assay of Malic Dehydrogenase (EC 1.1.1.37)

This is a protocol to be used for all Malic Dehydrogenase products.

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