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M2909

Sigma-Aldrich

Murashige and Skoog Modified Basal Salt Mixture

powder, suitable for plant cell culture

Synonym(s):

Murashige and Skoog Modified Basal Salt Mixture, w/o Ammonium Nitrate

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10 L
$57.70

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

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form

powder

Quality Level

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

2-8°C

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This Item
M5524M6899S6765
technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

form

powder

form

powder

form

powder

form

fine powder

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Application

Murashige and Skoog Modified Basal Salt Mixture has been used:
  • to promote root development for internode stem suckers[1]
  • to promote shoot growth for the calluses[2]
  • for low nitrogen (LN) treatment, for the growth of Arabidopsis thaliana[3]

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M2909) contain the macronutrients and micronutrients of the original classic formulation without ammonium nitrate. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
Without NH4NO3
With the macro- and micronutrients described by Murashige and Skoog (1962).

Media Formulation

pictograms

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Warning

hcodes

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Callus induction and plant regeneration from mature embryos of a diverse set of wheat genotypes
Zale JM, et al.
Plant Cell, Tissue and Organ Culture, 76(3), 277-281 (2004)
Iron homeostasis alteration in transgenic tobacco overexpressing ferritin
Van WO, et al.
The Plant Journal, 17(1), 93-97 (1999)
Interactions of abscisic acid and sugar signalling in the regulation of leaf senescence
Pourtau N, et al.
Planta, 219(5), 765-772 (2004)
O Van Wuytswinkel et al.
The Plant journal : for cell and molecular biology, 17(1), 93-97 (1999-03-09)
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the
Gabriele B Monshausen et al.
The Plant cell, 21(8), 2341-2356 (2009-08-06)
Mechanical stimulation of plants triggers a cytoplasmic Ca(2+) increase that is thought to link the touch stimulus to appropriate growth responses. We found that in roots of Arabidopsis thaliana, external and endogenously generated mechanical forces consistently trigger rapid and transient

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