M2933

Sigma-Aldrich

MES hydrate

BioPerformance Certified, suitable for cell culture, ≥99.5%

Synonym(s):
4-Morpholineethanesulfonic acid, 2-(N-Morpholino)ethanesulfonic acid hydrate
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

assay

≥99.5%

application(s)

cell culture | mammalian: suitable

impurities

endotoxin and Total Aerobic Microbial Count, tested

useful pH range

5.5 - 6.7

pKa 

6.1

Featured Industry

Diagnostic Assay Manufacturing

foreign activity

DNase, RNase, protease, none detected

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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General description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Application

MES Hydrate has been used:
  • To suspend cells before autophagic induction studies
  • In the preparation of Murashige and Skoog growth medium for the growth of Arabidopsis thaliana seedlings
  • In the conjugation of hybridization probes to beads before PCR amplification
  • To treat fibroblast-derived matrix before conjugation with heparin for use as a vascular endothelial growth factor delivery platform

Packaging

25, 100, 500 g in poly bottle
1 kg in poly bottle

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8°C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES products with the MES specification table.
Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Multiplex assay for subtyping avian influenza A viruses by cDNA hybridization and adapter-mediated amplification.
Applied Microbiology and Biotechnology, 100 (20), 8809-8818 (2016)
Autophagic or necrotic cell death in the absence of caspase and bcl-2 family members.
Lam, David, et al.
Biochemical and biophysical research communications, 363 (3), 536-541 (2007)
Fibroblast-derived matrix (FDM) as a novel vascular endothelial growth factor delivery platform.
Du, Ping, et al.
J. Controlled Release, 194, 122-129 (2014)
Dawson, R.M.C. et al.
Data for Biochemical Research, 410-410 (1987)
Protocol: An improved high-throughput method for generating tissue samples in 96-well format for plant genotyping (Ice-Cap 2.0).
Plant methods, 3 (1), 8-8 (2007)

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