The M3168 product does not require a stop solution. 4MUP is a fluorescent substrate. Adding a stop solution to 4MUP, such as an acidic solution like 2M Sulfuric Acid, would greatly reduce or eliminate the fluorescence. While stop solutions are typically added to substrates like pNPP, TMB, or OPD, they are not required when working with product M3168. The Product Information Sheet for M3168 can be downloaded from the website, where it mentions that the solution is ready to use and nothing else should be added to it.
M3168
4-Methylumbelliferyl phosphate
alkaline phosphatase substrate, liquid
Synonym(s):
4-methylumbelliferone phosphate Liquid Substrate System
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About This Item
CAS Number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
EC Number:
222-137-7
MDL number:
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Product Name
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System, liquid
SMILES string
CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12
InChI key
BCHIXGBGRHLSBE-UHFFFAOYSA-N
InChI
1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)
form
liquid
storage temp.
2-8°C
Quality Level
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form liquid | form powder or crystals | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.
Application
4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.
Analysis Note
4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups
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Instructions
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Which stop solution is the best option for AP-4MUP ELISA? Please advise.
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