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4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System


4-methylumbelliferone phosphate Liquid Substrate System
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General description

4-Methylumbelliferyl phosphate (4-MUP) is a substrate for alkaline phosphatase which forms the soluble fluorescent substance methylumbelliferone. 4-MUP Liquid Substrate System combines 4-MUP and buffer in a ready-to-use reagent.


4-Methylumbelliferyl phosphate (4-MUP) Liquid Substrate System has been used as a fluorescent substrate for alkaline phosphate in enzyme linked immunosorbent assay (ELISA). It has also been used in secreted embryonic alkaline phosphatase (SEAP) activity assay.

Analysis Note

4-MUP fluorescence can be measured with excitation at 360 nm and emission at 440 nm. The fluorescent end product may also be observed using a UV light source.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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As part of a program to develop better PTP1B fluorogenic substrates that more closely mimic the functionality found in the natural substrate, we have prepared and evaluated nine novel analogs of 4-methylumbelliferone phosphate (MUP) with a variety of additional groups

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