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M3546

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

Synonym(s):

protein standards

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1 vial
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$266.00
5 x 1 vial
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$1,050.00

About This Item

NACRES:
NA.25
UNSPSC Code:
41105337
Biological source:
bovine
Form:
liquid
Storage temp.:
−20°C

$266.00

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biological source

bovine

Quality Level

100, 200

form

liquid

usage

 vial sufficient for 400 applications on large gels (16 × 18 cm),  vial sufficient for 800 mini-gels (10 × 10 cm)

packaging

vial of 200 μL

storage temp.

−20°C

Application

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600) has been used as a protein marker in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.[1]

Biochem/physiol Actions

The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading.

Physical form

Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol

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This Item
C6210B2787SDS7B2
Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

Sigma-Aldrich

M3546

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

Color Marker Ultra-low Range (M.W. 1,060-26,600)

Supelco

C6210

Color Marker Ultra-low Range (M.W. 1,060-26,600)

Biotinylated Molecular Weight Marker mol wt 6,500-180,000 Da

Sigma-Aldrich

B2787

Biotinylated Molecular Weight Marker

Sigma-Aldrich

Sigma-Aldrich

SDS7B2

Prestained Molecular Weight Marker

biological source

bovine

biological source

-

biological source

-

biological source

-

Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

Quality Level

200, 400

form

liquid

form

liquid

form

lyophilized powder

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

packaging

vial of 200 μL

packaging

-

packaging

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

packaging

-

usage

 vial sufficient for 400 applications on large gels (16 × 18 cm),  vial sufficient for 800 mini-gels (10 × 10 cm)

usage

sufficient for 20 applications (on 10 cm × 10 cm mini gels)

usage

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

usage

-

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000477198
0000477198
0000403949
0000403949
0000421070

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Protocols

Cationic Polymers HPLC on TSKgel® PWXL-CP Columns

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
Morassutti C, et al.
Febs Letters, 519, 141-146 (2002)
Endostatin enhances radioresponse in breast cancer cells via alteration of substance P levels.
Arslan Aydemir E, et al.
Oncology Letters, 2, 879-886 (2011)
Raquel Soares Oliveira et al.
Toxins, 12(6) (2020-06-19)
The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin
View All Related Papers

Global Trade Item Number

SKUGTIN
M3546-5X1VL04061834050391
M3546-1VL04061834050384

Questions

  1. Can I use it for Coomassie blue stain SDS Page (16.5%)? Just to confirm: Is it for 1kDa to 26kDa range of proteins?

    1 answer

    1. Technically, the Ultra Low Range Molecular Weight Marker (M.W. 1,060–26,600), Sigma Catalog Number M3546, can be used with Coomassie Blue staining on a 16.5% SDS-PAGE gel. Since we do not have compatibility data with Coomassie blue staining, the customers will need to validate this on their behalf.

      Each vial of marker contains 200 ml of a mixture of six peptides and proteins (see Table 1) in 100 mM
      Tris-HCl, pH 8.5, 4 mM EDTA, 3 mM sodium azide, and 40% glycerol. The 2x sample buffer is a solution of 100 mM Tris-HCl, pH 6.8, 1% SDS, 4% 2-mercaptoethanol, 0.02% Brilliant Blue G, and 24% glycerol.

      Peptide and Protein Mixture in M3546
      Peptide/Protein Molecular Mass (Da)
      Triosephosphate Isomerase 26,600
      from rabbit muscle
      Myoglobin from horse heart 17,000
      a-Lactalbumin from bovine milk 14,200
      Aprotinin from bovine lung 6,500
      Insulin Chain B, oxidized, bovine 3,496
      Bradykinin 1,060
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/126/951/m3546dat.pdf

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