M3550

Sigma-Aldrich

Anti-MAP Kinase Activated Protein Kinase-2 (MAPKAPK-2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 45 kDa

species reactivity

human, bovine, mouse

application(s)

microarray: suitable
western blot: 1:2,000 using MDBK whole cell extract

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)

General description

MAP Kinase Activated Protein Kinase-2 (MAPKAPK-2) is a protein that belongs to Ser/Thr protein kinase family and is activated by p38 MAPK in response to several signals like mitogenic and stress stimuli. It has a crucial role in phosphorylating LIMK1 and hence plays a vital function in VEGF-A-induced actin reorganization, migration, and tubule formation of endothelial cells. Anti-MAP Kinase Activated Protein Kinase-2 (MAPKAPK-2) antibody can be used to study the differential tissue expression of MAPKAPK-2 and its intracellular localization in normal and neoplastic tissues. Rabbit anti-MAP Kinase Activated Protein Kinase-2 (MAPKAPK-2) antibody reacts specifically with human MAPKAPK-2protein (45 kD).

Immunogen

A synthetic peptide corresponding to amino acids 343-358 of human MAP kinase activated protein kinase 2 (MAPKAPK-2), coupled to KLH. The sequence is identical in mouse MAPKAPK-2.

Application

Anti-MAP Kinase Activated Protein Kinase-2 (MAPKAPK-2) antibody can be used in western blotting (diluted 1:2000) and microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
E Cano et al.
Oncogene, 12(4), 805-812 (1996-02-15)
We recently showed that EGF and anisomycin activate two kinases, p45 and p55, whose distinguishing feature is that their detection in in-gel kinase assays is enhanced by copolymerised poly-Glu/Tyr or poly-Glu/Phe (Cano E, Hazzalin CA and Mahadevan LC, Mol. Cell....
Miho Kobayashi et al.
The EMBO journal, 25(4), 713-726 (2006-02-04)
Vascular endothelial growth factor-A (VEGF-A) induces actin reorganization and migration of endothelial cells through a p38 mitogen-activated protein kinase (MAPK) pathway. LIM-kinase 1 (LIMK1) induces actin remodeling by phosphorylating and inactivating cofilin, an actin-depolymerizing factor. In this study, we demonstrate...
A Cuenda et al.
FEBS letters, 364(2), 229-233 (1995-05-08)
A class of pyridinyl imidazoles inhibit the MAP kinase homologue, termed here reactivating kinase (RK) [Lee et al. (1994) Nature 372, 739-746]. We now show that one of these compounds (SB 203580) inhibits RK in vitro (IC50 = 0.6 microM)...
J Rouse et al.
Cell, 78(6), 1027-1037 (1994-09-23)
MAPK-activated protein kinase-2 (MAPKAP kinase-2) is activated in vitro by the p42 and p44 isoforms of MAPK (p42/p44MAPK). In several cell lines, however, MAPKAP kinase-2 is activated by sodium arsenite, heat shock, or osmotic stress and not by agonists that...
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