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M3682

Sigma-Aldrich

Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal

clone ERK-PY193, purified from hybridoma cell culture

Synonym(s):
Anti-pY-ERK
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

ERK-PY193, monoclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

species reactivity

rat, human

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

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conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

ERK-PY193, monoclonal

clone

ERK-PT115, monoclonal

clone

ERK-NP2, monoclonal

clone

1B3B9, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa

mol wt

antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa

mol wt

antigen, ERK-1 44 kDa, antigen, ERK-2 42 kDa

mol wt

antigen 42 kDa

General description

Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.

Specificity

The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

Immunogen

synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Application

Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot-blot
  • immunocytochemistry
  • western blotting
  • immunofluorescence

Biochem/physiol Actions

MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Prepared from a culture supernatant of bioreactor grown hybridoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Mammalian MAP kinase signalling cascades
Chang L and Karin M
Nature, 410(6824), 37-37 (2001)
Sudhakaran Prabakaran et al.
Biophysical journal, 106(12), 2720-2728 (2014-06-19)
Mathematical models are extensively employed to understand physicochemical processes in biological systems. In the absence of detailed mechanistic knowledge, models are often based on network inference methods, which in turn rely upon perturbations to nodes by biochemical means. We have
Monophosphothreonyl extracellular signal-regulated kinases 1 and 2 (ERK1/2) are formed endogenously in intact cardiac myocytes and are enzymically active.
Sugden PH, et al.
Cellular Signalling, 23(2), 468-477 (2011)
Paradoxical results in perturbation-based signaling network reconstruction
Prabakaran S, et al.
Biophysical Journal, 106(12), 2720-2728 (2014)
Peter H Sugden et al.
Cellular signalling, 23(2), 468-477 (2010-11-04)
ERK1 and ERK2 (ERK1/2) are central to the regulation of cell division, growth and survival. They are activated by phosphorylation of the Thr- and the Tyr- residues in their Thr-Glu-Tyr activation loops. The dogma is that dually-phosphorylated ERK1/2 constitute the

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