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M3696

Sigma-Aldrich

Anti-MAP2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-Microtubule associated protein 2
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~280 kDa
antigen ~72 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

application(s)

flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

General description

Microtubule associated protein 2 (MAP2) is a neuron specific protein, which is highly expressed in the central nervous system. It is the member of cytoskeletal proteins. The gene is located on human chromosome 2q34.
MAP2 is a microtubule-associated protein that regulates the structure and function of the cytoskeleton. The protein also modulates morphogenesis of neurons and cell proliferation. Interactions of MAP2 with F-actin have been implicated in neurite initiation and microtubular re-organization. Decreased levels of MAP2 have been associated with transmissible spongiform encephalopathies . Anti-MAP2 antibody is specific for MAP2 in human, mice and rats.

Immunogen

synthetic peptide corresponding to residues 2-15 of human MAP2.

Application

Anti-MAP2 antibody is suitable for use in immunohistochemistry (1:500 using Zamboni′s fixative-fixed, frozen mouse spinal cord and thalamus sections) , flow cytometry (1:200-1:500), immunoblotting (approx. 72 kDa and 280 kDa) and western blot (1:500-1:1,000).
Anti-MAP2 antibody produced in rabbit has been used in immunocytochemistry.

Biochem/physiol Actions

Microtubule associated protein 2 (MAP2) plays a critical role in the formation of dendritic protrusions in astrocytes and oligodendrocytes. It maintains the homeostasis of microtubules. The protein plays an important role in neuronal plasticity.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Cory C Toth et al.
Molecular pain, 6, 16-16 (2010-03-20)
Despite the frequency of diabetes mellitus and its relationship to diabetic peripheral neuropathy (DPN) and neuropathic pain (NeP), our understanding of underlying mechanisms leading to chronic pain in diabetes remains poor. Recent evidence has demonstated a prominent role of microglial...
Specific binding of dehydroepiandrosterone to the N terminus of the microtubule-associated protein MAP2
Laurine E, et al.
The Journal of Biological Chemistry, 278(32), 29979-29986 (2003)
Transcriptional upregulation of microtubule-associated protein 2 is involved in the protein kinase A-induced decrease in the invasiveness of glioma cells
Zhou Y, et al.
Neuro-Oncology, 17(12), 1578-1588 (2015)
Rouhollah Habibey et al.
Scientific reports, 7(1), 8558-8558 (2017-08-19)
Due to their small dimensions, electrophysiology on thin and intricate axonal branches in support of understanding their role in normal and diseased brain function poses experimental challenges. To reduce experimental complexity, we coupled microelectrode arrays (MEAs) to bi-level microchannel devices...
Leif Dehmelt et al.
Genome biology, 6(1), 204-204 (2005-01-12)
Microtubule-associated proteins (MAPs) of the MAP2/Tau family include the vertebrate proteins MAP2, MAP4, and Tau and homologs in other animals. All three vertebrate members of the family have alternative splice forms; all isoforms share a conserved carboxy-terminal domain containing microtubule-binding...

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