This product is supplied by the ATCC. The growth phase at the time of harvest is not reported by the supplier. In general, in order to maximize production and viability, cells are in log phase or beginning stationary phase at the time of harvest.
M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Synonym(s):
Micrococcus luetus
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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
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lyophilized cells
suitability
suitable for substrate for the assay of lysozyme
storage temp.
−20°C
Quality Level
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This Item | M0508 | SRE0018 | N3755 |
|---|---|---|---|
| suitability suitable for substrate for the assay of lysozyme | suitability - | suitability - | suitability - |
| form lyophilized cells | form lyophilized cells | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 300 |
Application
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. [1] It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles. [2]
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes. [3]
Biochem/physiol Actions
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Analysis Note
Contains polynucleotide phosphorylase.
Other Notes
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Generation of a reference transcriptome for evaluating rainbow trout responses to various stressors.
Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Tracking molecular recognition at the atomic level with a new protein scaffold based on the OB-fold.
John D Steemson et al.
PloS one, 9(1), e86050-e86050 (2014-01-28)
The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin
T Yoshimoto et al.
Journal of biochemistry, 78(2), 253-259 (1975-08-01)
Using lysozyme-lysate of Micrococcus lysodeikticus cell wall coupled with Sepharose, several bacteriolytic enzymes were purified from crude preparations of animal and microbial origin. Quail egg-white, human milk and salivary lysozymes [EC 3.2.1.17] were adsorbed onto the adsorbent at pH 5-7
Ingrid Bourgeois et al.
FEMS microbiology letters, 290(1), 105-113 (2008-11-26)
The nucleotide sequence of atlL, a gene encoding a putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp gene encodes a protein, AtlL, that appears as a putative bifunctional autolysin with a 29-amino
Protocols
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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From which growth phase are the bacteria harvested?
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