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M3900

Sigma-Aldrich

Murashige and Skoog Vitamin Solution

1000 ×, liquid, suitable for plant cell culture

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NACRES:
NA.72

sterility

sterile-filtered

form

liquid

concentration

1000 ×

technique(s)

cell culture | plant: suitable

color

colorless

pH

5.5-7.5

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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This Item
M0529M0654B5282
form

liquid

form

liquid

form

liquid

form

liquid

concentration

1000 ×

concentration

10 ×

concentration

10 ×

concentration

50 ×

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

Application

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Use at a concentration of one ml per liter of prepared medium to achieve the proper final concentration.

Components

Solution contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

Preparation Note

Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Vitamin powder (M3900) can be combined with Murashige and Skoog micro/macro-nutrients to generate basal media which when supplemented with organics, agar, auxins and cytokinins yield complete medium for growth plant tissue culture. The M3900 Solution is a 1000x concentrate which contains (mg/ml): 2.0 glycine, 100.0 myo-inositol, 0.50 nicotinic acid, 0.50 pyridoxine hydrochloride, 0.10 thiamine hydrochloride.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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O Van Wuytswinkel et al.
The Plant journal : for cell and molecular biology, 17(1), 93-97 (1999-03-09)
Intracellular iron concentration requires tight control and is regulated both at the uptake and storage levels. Our knowledge of the role that the iron-storage protein ferritins play in plants is still very limited. Overexpression of this protein, either in the
Ting Li et al.
Nature communications, 10(1), 726-726 (2019-02-15)
In plants mechanical signals pattern morphogenesis through the polar transport of the hormone auxin and through regulation of interphase microtubule (MT) orientation. To date, the mechanisms by which such signals induce changes in cell polarity remain unknown. Through a combination
Saxena et al.
Plant science : an international journal of experimental plant biology, 155(2), 133-140 (2000-05-18)
Efficient protocols have been established for both direct and indirect regeneration of plants in Pelargonium graveolens Indian cultivar Hemanti (Algerian type). Murashige and Skoog's (MS) medium [T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco

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