M3946

Sigma-Aldrich

Anti-MNK1 antibody produced in rabbit

~1.4 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-MAPK signal-integrating serine/threonine kinase 1
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~40 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.4 mg/mL

application(s)

western blot: 1-2 μg/mL using lysates of HEK-293T cells transfected with human Mnk1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... MKNK1(8569)

Related Categories

General description

MNK1 is a serine-threonine kinase that is activated by ERK or p38 MAPK signaling cascades and modulates translation through eIF4E phosphorylation. Human MNK1 has two C-terminal splice variants, namely, MNK1a and MNK1b. While MNK1a is expressed in the cytoplasm, MNK1b is mainly localized at the nucleus . Anti-MNK1 antibody is specific for human Mnk1 (40 kDa). In immunoblotting, staining of the Mnk1 band is specifically inhibited by the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 12-27 of human MNK1, conjugated to KLH.

Application

Anti-MNK1 antibody is suitable for use in western blot (1-2 μg/mL using lysates of HEK-293T cells transfected with human Mnk1).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Ana O'Loghlen et al.
Experimental cell research, 299(2), 343-355 (2004-09-08)
In this paper, we report the identification and molecular characterization of a splice variant of human Mnk1 which has been named as Mnk1b. Human Mnk1b mRNA is homologous to human Mnk1 mRNA but lacking a region corresponding to exon 19...
Maria Buxade et al.
Frontiers in bioscience : a journal and virtual library, 13, 5359-5373 (2008-05-30)
The human MAP kinase-interacting kinases (or MAP kinase signal-integrating kinases), Mnks, comprise a group of four proteins derived from two genes (Gene symbols: MKNK1 and MKNK2) by alternative splicing. Mnk1a/b differ at their C-termini, as do Mnk2a/2b: in each case...
R Fukunaga et al.
The EMBO journal, 16(8), 1921-1933 (1997-04-15)
We have developed a novel expression screening method for identifying protein kinase substrates. In this method, a lambda phage cDNA expression library is screened by in situ, solid-phase phosphorylation using purified protein kinase and [gamma-32P]ATP. Screening a HeLa cDNA library...
Takeshi Ueda et al.
Molecular and cellular biology, 24(15), 6539-6549 (2004-07-16)
Mnk1 and Mnk2 are protein kinases that are directly phosphorylated and activated by extracellular signal-regulated kinase (ERK) or p38 mitogen-activated protein (MAP) kinases and implicated in the regulation of protein synthesis through their phosphorylation of eukaryotic translation initiation factor 4E...
Haizhong Feng et al.
The Journal of clinical investigation, 124(9), 3741-3756 (2014-07-26)
Aberrant activation of EGFR in human cancers promotes tumorigenesis through stimulation of AKT signaling. Here, we determined that the discoidina neuropilin-like membrane protein DCBLD2 is upregulated in clinical specimens of glioblastomas and head and neck cancers (HNCs) and is required...

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