MilliporeSigma
All Photos(1)

M4401

Sigma-Aldrich

Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse

clone MY-21, ascites fluid

Synonym(s):
Myosin Light Chain Antibody, Myosin Light Chain Antibody - Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MY-21, monoclonal

mol wt

antigen 20 kDa

contains

15 mM sodium azide

species reactivity

chicken, pig, rabbit, bovine, human

technique(s)

indirect immunofluorescence: 1:200 using human or chicken fibroblasts
western blot: suitable using denatured and reduced myosin of muscle

isotype

IgM

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... MYLK2(396356)
human ... MYLK2(85366)

General description

Monoclonal Anti-Myosin (Light Chains 20 kDa) (mouse IgM isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin is a 500,000 Da protein known to interact with identical heavy chains (200,000 daltons each) and four light chains (15,000-20,000 daltons). Myosin molecules consist of two major regions: tail (rod) and head regions.

Specificity

Monoclonal Anti-Myosin reacts best with the 20,000 dalton light chains of chicken gizzard, intestine or pig stomach myosin (SDS heat denatured and reduced).

Immunogen

chicken lens membrane.

Application

Monoclonal Anti-Myosin (Light Chains 20 kDa) antibody produced in mouse has been used in:
  • western blot analysis
  • fluorescence immunohistochemistry
  • confocal microscopy

Monoclonal anti-myosin (Light Chains 20 kDa) antibody can be used in immunoblotting and western blotting.

Biochem/physiol Actions

Myosin is a cytoskeletal protein responsible for muscle contraction, muscle filament sliding and regulation of ATPase activity. Monoclonal anti-myosin (Light Chains 20 kDa) antibody diluted 1:200 can be used to detect MLC20. It is also useful in staining (1:10,000) 2D slab gels. Anti-myosin(Light Chains 20 kDa) antibody reacts specifically with 20 kDa light chain of chicken gizzard, chicken intestine and pig stomach myosin which is SDS, heat denatured and reduced but not with intact myosin molecule in its native state.
The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Numerous investigators have shown that actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. Thick filaments consist mainly of myosin molecules. Myosin is an enzyme (ATPase) and its activity is the immediate source of the free energy that drives muscle contraction. Myosin binds to the polymerized form of actin, the major constituent of the thin filament.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service