M4439

Anti-c-Myc antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• Synonym(s): Anti-c-Myc
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: buffered aqueous solution
• mol wt: 62 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:5000 using c-myc tagged fusion protein
• UniProt accession no.: P01106
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... MYC(4609)

Description

General description

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.

c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper.[1] Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family.[2] It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA.[1] This gene is localized to human chromosome 8q24.[3]

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation,[4] immunoblotting,[5][6][7] ELISA, immunofluorescence,[8][9] microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).[10][11]

Biochem/physiol Actions

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability.[12] This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis.[13] Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4⁺ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation.[1] It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).[14]

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Working dilution: 1:5000

Other Notes

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Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable