M4439

Anti-c-Myc antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• Synonym(s): Anti-c-Myc
• NACRES: NA.56

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• form: buffered aqueous solution
• mol wt: 62 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:5000 using c-myc tagged fusion protein
• UniProt accession no.: P01106
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... MYC(4609)

Description

General description

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.

c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

Immunogen

synthetic peptide of the human p62^c-Myc protein.

Application

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

Biochem/physiol Actions

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4⁺ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Working dilution: 1:5000

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable