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M4642

Sigma-Aldrich

Minimum Essential Medium Eagle

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
MEM
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
M1018M0644M8042
vibrant-m

M4642

Minimum Essential Medium Eagle

vibrant-m

M1018

Minimum Essential Medium Eagle

vibrant-m

M0644

Minimum Essential Medium Eagle

vibrant-m

M8042

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

glucose: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes
Earle’s salts (5% CO2): no
NaHCO3: no
phenol red: yes
sodium pyruvate: no
stable glutamine: no
HEPES: no

components

phenol red: yes
NaHCO3: no
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

MEM, classic media, classical media, FBS

MEM, classic media, classical media, FBS

classic media, classical media, FBS, hybrid cells

classic media, classical media, MEM-alpha, classic media, classical media, FBS

General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has high concentrations of amino acids, so the medium closely approximates the protein concentration of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has increased the applicability of this medium. This medium lacks calcium that permits the growth of cells in suspension. Modification of the original MEM formulation. Earle′s salts have been replaced with Hanks′ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Application

Minimum Essential Medium Eagle (MEM) has been used:

  • as a component of the Hank′s MEM (HMEM)-Pipes buffer to induce anaphylatoxin induced-ear swelling in mice
  • as a component of the slice culture medium (SCM) for the incubation of mouse hippocampal slice cultures
  • to culture Müller cells from guinea pigs

Quantity

Formulated to contain 10.6 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium
Iwona M G, et al.
Journal of Neuroimmunology, 161, 49-60 (2005)
Mast cell anaphylatoxin receptor expression can enhance IgE-dependent skin inflammation in mice
Beatrix S, et al.
The Journal of Allergy and Clinical Immunology, 131(2), 541-548 (2013)
Anthony Dudilot et al.
Current biology : CB, 30(7), 1167-1176 (2020-02-23)
Synaptic plasticity, with its two most studied forms, long-term potentiation (LTP) and long-term depression (LTD), is the cellular mechanism underlying learning and memory. Although it has been known for two decades that bidirectional synaptic plasticity necessitates a corresponding bidirectional regulation
John K Mich et al.
Cell reports, 34(13), 108754-108754 (2021-04-01)
Viral genetic tools that target specific brain cell types could transform basic neuroscience and targeted gene therapy. Here, we use comparative open chromatin analysis to identify thousands of human-neocortical-subclass-specific putative enhancers from across the genome to control gene expression in
Simona Giunta et al.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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