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M4655

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
MEM
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

HEPES: no
NaHCO3: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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