M5057

Sigma-Aldrich

MES sodium salt

BioReagent, ≥99% (titration), crystalline

Synonym(s):
4-Morpholineethanesulfonic acid sodium salt, 2-(N-Morpholino)ethanesulfonic acid sodium salt
Empirical Formula (Hill Notation):
C6H12NNaO4S
CAS Number:
Molecular Weight:
217.22
Beilstein/REAXYS Number:
3765682
EC Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioReagent

assay

≥99% (titration)

form

crystalline

pH

8.9-10.1 (1% in water)

useful pH range

5.5 - 6.7

pKa 

6.1

solubility

water: 0.5 g/mL, clear, colorless

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Na+].[O-]S(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1

InChI key

IRHWMYKYLWNHTL-UHFFFAOYSA-M

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General description

MES is one of the so-called "Good" buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Application

MES sodium salt has been used:
  • To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNA
  • To equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization

Packaging

10, 25, 100 g in poly bottle

Preparation Note

MES Sodium is soluble in water, giving a clear colourless solution at 25g in 50mL water (approximately 2.3M). The pH of a solution should be between 9 and 10 depending on concentration. Solutions should be stable at 2-8°C for months. Sterilization should be by filteration through 0.2μm filters. Autoclaving is not recommended. If buffers must be nuclease-free, it is best to treat the water first, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Easily compare specifications for MES sodium salt products with the MES sodium salt specification table.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Dawson, RMC et al.
Data for Biochemical Research, 410-410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)
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Journal of molecular biology, 321(5), 879-889 (2002-09-11)
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