M5287

Sigma-Aldrich

MES hydrate

≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra

Synonym(s):
4-Morpholineethanesulfonic acid, 2-(N-Morpholino)ethanesulfonic acid hydrate
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

Quality Level

product line

BioXtra

assay

≥99.5% (titration)

form

crystalline powder

impurities

Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.05%

pH

2.5-4.0 (0.5 M in H2O)

useful pH range

5.5 - 6.7

pKa 

6.1

solubility

H2O: 0.5 M, clear, colorless

anion traces

chloride (Cl-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.002%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.020 at 280 in H2O at 0.5 M
≤0.025 at 260 in H2O at 0.5 M

storage temp.

room temp

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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General description

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Application

MES Hydrate has been used:
  • As a component of extraction buffer in the extraction of the enzyme BACE1
  • As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblasts
  • As a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beads

Packaging

50, 250 g in poly bottle

Preparation Note

A buffer using MES can be prepared by titrating with NaOH to the desired pH. Alternatively, stock solutions of MES and MES sodium salt can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare buffers of a given pH have been published. MES is not recommended for buffering at pH 7.4; other buffers should be considered.

Storage and Stability

Solutions are stable at 2-8 C for months. Sterilize by filtration through 0.2uM filters. Autoclaving is not recommended for any sulfonic acid buffer. If buffers must be nuclease-free, treat the water first, and then add the buffer after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown.

Other Notes

Solubility: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. Similar product MES hydrate M8250 is tested at 20g/80mL water, or 1.3M. The pH of a solution should be between 2.5 and 5, depending on the concentration. A saturated solution at 0°C is approximately 0.65M.
Easily compare specifications for MES products with the MES specification table.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

High-sensitivity detection and quantitative analysis of native protein-protein interactions and multiprotein complexes by flow cytometry.
Schrum AG, et al.
Science Signaling, 2007 (389), pl2-pl2 (2007)
5, 10-methenyltetrahydrofolate synthetase deficiency causes a neurometabolic disorder associated with microcephaly, epilepsy, and cerebral hypomyelination.
Rodan, LH et al.
Molecular Genetics and Metabolism, 125, 118-126 null
The Swedish APP mutation alters the effect of genetically reduced BACE1 expression on the APP processing.
Rabe S, et al.
Journal of Neurochemistry, 119 (1), 231-239 (2011)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, CMH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
Good, Norman E. et al.
Biochemistry, 5, 467- 477 (1966)

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