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Mineral oil

light oil, suitable for mouse embryo cell culture, BioXtra

CAS Number:
EC Number:
MDL number:



product line



light oil

storage condition

protect from light


cell culture | embryo: suitable

refractive index

n20/D 1.467 (lit.)


0.82-0.88 g/mL at 25 °C



InChI key


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General description

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.


Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Storage Class Code

10 - Combustible liquids



Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Mineral Oil Overlay for Conservation of Plant Tissue Cultures
Caplin SM
American Journal of Botany, 46(5), 324-329 (1959)
Bei Zhao et al.
Scientific reports, 7(1), 11319-11319 (2017-09-14)
A microfluidic chip with a controllable and integrated piezoelectric pump was proposed and demonstrated, where the pump was designed as a micro-actuator based on polyvinylidene fluoride (PVDF) organic piezoelectric film. In this case, the pump should integrate with the microfluidics
Hanyoup Kim et al.
Optics express, 17(1), 218-227 (2009-01-09)
We report the successful application of low-power (approximately 30 mW) laser radiation as an optical heating source for high-speed real-time polymerase chain reaction (PCR) amplification of DNA in nanoliter droplets dispersed in an oil phase. Light provides the heating, temperature
Hanyoup Kim et al.
Lab on a chip, 9(9), 1230-1235 (2009-04-17)
We report high-speed real-time PCR performed on an unmodified disposable polystyrene Petri dish. The reaction cycle relies solely on an infrared laser for heating; no conventional heater is required. Nanoliter droplets of PCR mixture as water-in-oil emulsions printed in an
Xiyan Wang et al.
Journal of clinical laboratory analysis, 34(11), e23498-e23498 (2021-01-20)
Efficiency of preantral follicle culture in vitro is low and is dependent on species, development stage, and follicle-stimulating hormone (FSH) concentration. Here, we optimized the preantral follicle in vitro culture system in mice. The primary follicles (PM follicles, 80-100 μm


Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture


Determination of Water Content in Mineral oil Using Karl Fischer Titration

Summary application report for analysis of moisture in Mineral oil

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