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M5524

Sigma-Aldrich

Murashige and Skoog Basal Salt Mixture (MS)

powder, suitable for plant cell culture

Synonym(s):

MS Basal Salts, MS0 Basal Salts, MSO Basal Salts

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$18.60
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$54.60
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$193.00

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.72

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form

powder

Quality Level

100

technique(s)

cell culture | plant: suitable

application(s)

agriculture

shipped in

ambient

storage temp.

2-8°C

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This Item
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Murashige and Skoog Basal Salt Mixture (MS) powder, suitable for plant cell culture

Sigma-Aldrich

M5524

Murashige and Skoog Basal Salt Mixture (MS)

Murashige and Skoog Modified Basal Salt Mixture powder, suitable for plant cell culture

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M2909

Murashige and Skoog Modified Basal Salt Mixture

Sigma-Aldrich

Sigma-Aldrich

M6899

Murashige and Skoog Basal Salts with minimal organics

Sigma-Aldrich

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M0529

Murashige and Skoog Basal Salt Micronutrient Solution

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

form

powder

form

powder

form

powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

400

General description

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and Kinetin (cytokinin/cell division promoter).

Application

Murashige and Skoog Basal Salt Mixture (MS) has been used to support the growth and regeneration of transformed tobacco cells.[1] It has also been used as a growth medium for germination of Arabidopsis thaliana (Col-0) seeds.

Preparation Note

Formulated to contain 4.3 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog Salt mixture (M5524) contains the macronutrients and micronutrients of the original classic formulation. It can be combined with M&S vitamins or Gamborg′s vitamins and supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients as described by Murashige and Skoog (1962).

Media Formulation

Other Notes

Storage
Powdered media are extremely hygroscopic and must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, or particulate matter throughout the powder; 3) insolubility; 4) pH change; or 5) inability to promote growth when properly used.

Media Preparation

related product

Product No.
Description
Pricing

pictograms

Flame over circleExclamation mark
GHS03,GHS07

signalword

Warning

hcodes

H272,H319

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000467674
0000467674
0000467666
0000467666
0000389087

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Tohru Ariizumi et al.
The Plant cell, 20(9), 2447-2459 (2008-10-02)
This article presents evidence that DELLA repression of gibberellin (GA) signaling is relieved both by proteolysis-dependent and -independent pathways in Arabidopsis thaliana. DELLA proteins are negative regulators of GA responses, including seed germination, stem elongation, and fertility. GA stimulates GA
Chien-Yu Huang et al.
Plant physiology, 174(4), 2248-2260 (2017-06-15)
Plant cytosolic lipid droplets (LDs) are covered with a layer of phospholipids and oleosin and were extensively studied before those in mammals and yeast. Oleosin has short amphipathic N- and C-terminal peptides flanking a conserved 72-residue hydrophobic hairpin, which penetrates
Pyae Phyo et al.
Plant physiology, 175(4), 1593-1607 (2017-11-01)
At early stages of Arabidopsis (
Joanna Banasiak et al.
Frontiers in plant science, 11, 18-18 (2020-03-03)
Strigolactones (SLs) are plant-derived signaling molecules that stimulate the hyphal branching of arbuscular mycorrhizal fungi (AMF), and consequently promote symbiotic interaction between the fungus and the plant. Currently, our knowledge on the molecular mechanism of SL transport is restricted to
A gene encoding scots pine antimicrobial protein Sp-AMP2 (PR-19) confers increased tolerance against Botrytis cinerea in transgenic tobacco
Jaber E, et al.
Forests, 9(1), 10-10 (2017)
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Articles

Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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