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MilliporeSigma

M5528

Sigma-Aldrich

Methyl Blue

certified by the Biological Stain Commission, powder

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
249-352-9
MDL number:
MFCD00058509
UNSPSC Code:
12171500
PubChem Substance ID:
329818015
NACRES:
NA.47

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Product Name

Methyl Blue, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

technique(s)

microbe id | staining: suitable

solubility

H2O: 1 mg/mL, blue

εmax

≥700 at 594-610 nm in 5 mM HCl

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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1 of 4

This Item
95290M6900M9140
Methyl Blue certified by the BSC

Sigma-Aldrich

M5528

Methyl Blue

Methyl Blue suitable for microscopy

Sigma-Aldrich

95290

Methyl Blue

Methyl Blue powder or crystals

Sigma-Aldrich

M6900

Methyl Blue

Methylene blue certified by the BSC

Sigma-Aldrich

M9140

Methylene blue

grade

certified by the Biological Stain Commission

grade

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

grade

-

grade

certified by the Biological Stain Commission

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

technique(s)

-

technique(s)

microbe id | staining: suitable

form

powder

form

powder or crystals

form

powder or crystals

form

powder

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

solubility

H2O: 1 mg/mL, blue

solubility

water: 0.01 g/10 mL, blue to very deep blue

solubility

H2O: 1 mg/mL, blue

solubility

H2O: 4mg/4ml

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Application

Certified as aniline blue for use in Mallory′s connective tissue stain and as a substitute for fast green FCF in Gomori′s one-step trichrome procedure.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

>572.0 °F - Pensky-Martens closed cup

flash_point_c

> 300 °C - Pensky-Martens closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM7285
SHBM0391
MKCG1609
MKCF2706
SLBP6577V

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The purpose of this study was to investigate the biocompatibility of aniline and methyl blue in a well-established cell culture model and assess the staining properties of these dyes at the level of the internal limiting membrane (ILM) in human
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