This product is not assigned an expiration date. Products that have no expiration date are robust in nature and extremely stable in the original sealed container. These products will have no dating reported on either the label or the Certificate of Analysis but are covered by a warranty period of 1 year from the date of product shipment. The complete Product Dating statement may be reviewed at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf .
M5550
MEM Amino Acids (50x) solution
Without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
MEM Amino Acids mixture
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About This Item
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sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
Quality Level
1 of 4
This Item | M7145 | M0275 | M8042 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities endotoxin, tested | impurities endotoxin, tested | impurities endotoxin, tested | impurities endotoxin, tested |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 400 |
Application
Adding supplements of amino acids to media stimulates growth and prolongs the viability of cells in culture. MEM Amino Acids (50x) solution concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. It has been used in in vitro cultures,[1] co-cultures[2] and incubation media.[3]
Preparation Note
This product may contain a yellow hue if exposed to light. This is due to the presence of the amino acids tryosine and tryptophan in the solution. The change in color to the liquid solution has no affect to the quality or integrity of the product. For a complete list of components, please see the formulation sheet listed in the "Documents" section of the product page.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Geba KM, et al.
Fish Physiology and Biochemistry, 42(1), 365-377 (2016)
Paleerath Peerapen et al.
Biochimica et biophysica acta, 1864(6), 683-696 (2016-03-16)
Chronic K(+) deficiency can cause hypokalemic nephropathy associated with metabolic alkalosis, polyuria, tubular dilatation, and tubulointerstitial injury. However, effects of acute K(+) deficiency on the kidney remained unclear. This study aimed to explore such effects by evaluating changes in levels
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Effect of plasma progesterone on oocyte recovery, oocyte quality,
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and early in-vitro developmental competence of embryos in Bos
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Saad M, et al.
Animal Reproduction Science, 80-86 (2019)
Anne D Thuesen et al.
American journal of physiology. Renal physiology, 307(4), F445-F452 (2014-06-27)
Voltage-gated Ca(2+) (Cav) channels play an essential role in the regulation of renal blood flow and glomerular filtration rate (GFR). Because T-type Cav channels are differentially expressed in pre- and postglomerular vessels, it was hypothesized that they impact renal blood
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Related Content
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How long can this be stored in the refrigerator for?
1 answer-
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What are the concentrations of AAs?
1 answer-
The concentration of amino acids can be found in the formulation documentation, please view the formulation document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/210/498/m5550for.pdf.
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Can this be used for cell free expression on E.coli system?
1 answer-
This product is intended for use in mammalian cell culture, not for bacteria.
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Can Product M5550, MEM Amino Acids (50X) solution contain precipitates?
1 answer-
Product M5550, MEM Amino Acids (50X) has a specification as a clear solution. This product can have a slight precipitate that forms at 2-8°C. This precipitate will disappear when warmed to room temperature or when diluted to 1X. There is no affect on the product quality.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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