Yes, i is advised to use a white plate with a clear bottom for a luciferase assay. The primary difference between white and black plates lies in their reflective properties. White plates reflect light, maximizing light output signal, which is suitable for luminescent assays. On the other hand, black plates absorb light, reducing background and crosstalk, making them ideal for fluorescent assays. In multiplexed assays, using a white plate will maximize light output signal for the luminescent portion but may lead to higher crosstalk and background for the fluorescent part. Conversely, using a black plate will reduce fluorescent signal crosstalk and background, but will also reduce the luminescence signal by about 1 order of magnitude.
M5686
Greiner high and medium binding 96 well plates
flat bottom clear polystyrene wells (with micro-clear bottom), medium binding surface, non-sterile
Synonym(s):
96 multiwell plates, 96 well microplates, 96 well microtiter plates, 96 well strip plates
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40 EA
$327.00
$327.00
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40 EA
$327.00
About This Item
UNSPSC Code:
41122107
NACRES:
NB.22
Material:
black polystyrene
flat bottom clear polystyrene wells (with micro-clear bottom)
flat bottom clear polystyrene wells (with micro-clear bottom)
Size:
96 wells
Sterility:
non-sterile
Binding type:
medium binding surface
Feature:
lid: no
skirt (F-bottom)
skirt (F-bottom)
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$327.00
In StockDetails
material
black polystyrene
flat bottom clear polystyrene wells (with micro-clear bottom)
sterility
non-sterile
feature
lid: no
skirt (F-bottom)
packaging
case of 40
manufacturer/tradename
Greiner 655096
maximum volume
392 μL
size
96 wells
working volume
340 μL
binding type
medium binding surface
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This Item | M4936 | M4561 | M4686 |
|---|---|---|---|
| binding type medium binding surface | binding type medium binding surface | binding type high binding surface | binding type high binding surface |
| material black polystyrene , flat bottom clear polystyrene wells (with micro-clear bottom) | material black polystyrene , flat bottom black wells | material clear polystyrene , flat bottom clear wells | material flat bottom white wells, white polystyrene |
| manufacturer/tradename Greiner 655096 | manufacturer/tradename Greiner 655076 | manufacturer/tradename Greiner 655061 | manufacturer/tradename Greiner 655074 |
| feature lid: no, skirt (F-bottom) | feature lid: no, skirt (F-bottom) | feature lid: no, skirt (F-bottom) | feature lid: no, skirt (F-bottom) |
| working volume 340 μL | working volume 340 μL | working volume - | working volume 340 μL |
| size 96 wells | size 96 wells | size - | size 96 wells |
General description
FLUOTRAC™ 200 96W Microplate, well working volume 25-340 μL; total well volume 392 μL.
Polystyrene Multiwell plates from Greiner for immunoassay and high throughput screening applications. High binding plates are recommended for ELISAs. High binding denotes a binding capacity of 600 ng/cm2 and scatter of less than 10%. Optimal protein binding is variable and comparative tests between high and medium binding plates is suggested.
Other Notes
96 well flat (F) μClear® bottom; medium binding microplate, chimney style, no lid, alphanumeric well coding, length: 127.76 mm; width: 85.48 mm; curvature approx. 200 um.
Suitability
Black plates minimize background fluorescence. FLUOTRAC 200 binds up to 200 ng/cm2. Clear bottoms allow for monitoring thru the plate bottom. Free of detectable DNase/RNase, human DNA and pyrogens.
Legal Information
μClear is a registered trademark of Greiner Bio-One GmbH
FLUOTRAC is a trademark of Greiner Bio-One GmbH
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