M5899
Anti-Mouse IgG (whole molecule) antibody produced in goat
whole antiserum
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biological source
goat
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
secondary antibodies
clone
polyclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:70,000
quantitative precipitin assay: 3.2-4.8 mg/mL
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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Related Categories
1 of 4
This Item | M7023 | M2650 | I1011 |
|---|---|---|---|
| biological source goat | biological source rabbit | biological source goat | biological source goat |
| antibody form whole antiserum | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody | antibody form whole antiserum |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| technique(s) indirect ELISA: 1:70,000, quantitative precipitin assay: 3.2-4.8 mg/mL | technique(s) indirect ELISA: 1:80,000, quantitative precipitin assay: 2.0 mg/mL | technique(s) indirect ELISA: 1:15,000 | technique(s) indirect ELISA: 1:40,000, quantitative precipitin assay: 4.0 mg/mL |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Application
Anti-Mouse IgG (whole molecule) antibody produced in goat has been used as secondary antibody in:
- immunoblotting
- immunohistochemistry
- proteomic microarrays
Anti-Mouse IgG (whole molecule) antibody produced in goat has been used in:
- the preparation of immunochromatography strips for magneto-enzyme lateral flow immunoassay[1]
- immunochromatography[1]
- MYCN DNA-binding analysis (ChIP-seq)[2]
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Biochem/physiol Actions
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
The antiserum is determined to be immunospecific for mouse IgG by immunoelectrophoresis (IEP) against normal mouse serum and purified mouse IgG.
Physical form
Supplied as a liquid containing 15 mM sodium azide as preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Analysis Note
The antiserum has been treated to remove lipoproteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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David J Duffy et al.
Oncotarget, 7(37), 60310-60331 (2016-08-18)
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