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M5904

Sigma-Aldrich

Mineral oil

BioReagent, for molecular biology, light oil

CAS Number:
EC Number:
MDL number:
NACRES:
NA.52

Quality Level

grade

for molecular biology

product line

BioReagent

form

light oil

application(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.

Packaging

5, 500 mL in poly bottle

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Microfluidic flow-focusing in ac electric fields
Tan SH, et al.
Lab on a chip, 14(6), 1099-1106 (2014)
S Nathakarnkitkool et al.
Electrophoresis, 13(1-2), 18-31 (1992-01-01)
Capillary electrophoretic separations of double-stranded DNA fragments in the size range of 20-2200 base pairs were achieved in less than 20 min with the use of a Tris-borate buffer containing hydroxyethylcellulose. Analyses were carried out in both uncoated and phenylmethyl-coated...
Process Fluids for Abrasive Machining
Tribology of Abrasive Machining Processes, 531-585 (2004)
Xi-Ming Sun et al.
Current biology : CB, 30(7), 1217-1230 (2020-02-16)
Cell size varies during the cell cycle and in response to external stimuli. This requires the tight coordination, or "scaling," of mRNA and protein quantities with the cell volume in order to maintain biomolecule concentrations and cell density. Evidence in...
Construction of a full-length enriched and a 5'-end enriched cDNA library using the oligo-capping method.
Yutaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 221, 73-91 (2003-04-22)

Protocols

Determination of Water Content in Mineral oil Using Karl Fischer Titration

Summary application report for analysis of moisture in Mineral oil

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