M5904

Sigma-Aldrich

Mineral oil

BioReagent, for molecular biology, light oil

CAS Number:
EC Number:
MDL number:
NACRES:
NA.52

Quality Level

grade

for molecular biology

product line

BioReagent

form

light oil

application(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

suitability

suitable for overlaying aqueous reactions and centrifuge gradients

foreign activity

DNase, RNase, Protease, none detected

storage temp.

room temp

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oils are usually seen as a mixture of liquid hydrocarbons. It is derived from crude oil by distillation and refining.

Application

Mineral oil has been used:
  • to suspend the gels for capillary contraction assay
  • in polymerase chain reaction (PCR) reactions mixture to form a vapor barrier in order to prevent the evaporation of the reaction at elevated temperatures
  • as a continuous phase to prevent droplet coalescence in emulsification system

Mineral oil is suitable for use as an overlay to control evaporation and cross contamination of samples in a variety of molecular biology applications.

Packaging

5, 500 mL in poly bottle

Biochem/physiol Actions

Mineral oil delivers a better insulation strength, when compared to air. It enhances the elimination of heat from the windings, when used in a power transformer.

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A quantitative analysis of contractility in active cytoskeletal protein networks
Bendix PM, et al.
Biophysical Journal, 94(8), 3126-3136 (2008)
Electrical distribution and installation
Plant Engineer's Reference Book (2002)
C D Liapis et al.
Anticancer research, 25(3c), 2449-2452 (2005-08-06)
Carotid body (CB) is a highly specialized paraganglion originating from the neural crest ectoderm. CB paraganglion can be caused either by a genetic predisposition (hereditary paraganglia) or by chronic hypoxic stimulation. Germline mutations in any of the following genes: SDHD...
Construction of a full-length enriched and a 5'-end enriched cDNA library using the oligo-capping method.
Yutaka Suzuki et al.
Methods in molecular biology (Clifton, N.J.), 221, 73-91 (2003-04-22)
Paraffin wax as a vapor barrier for the PCR.
Sparkman DR
Genome Research, 2(2), 180-181 (1992)
Protocols
Summary application report for analysis of moisture in Mineral oil
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