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M5909

Sigma-Aldrich

IgM Isotype Control from murine myeloma

clone MOPC 104E, 200 μg/mL, buffered aqueous solution, purified immunoglobulin

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Synonym(s):
Mouse IgM Isotype Control from myeloma
MDL number:
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

MOPC 104E, monoclonal

form

buffered aqueous solution

concentration

200 μg/mL

technique(s)

flow cytometry: suitable

isotype

IgMλ

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
M5409M5284F6397
clone

MOPC 104E, monoclonal

clone

UPC-10, monoclonal

clone

MOPC 21, monoclonal

clone

MOPC 21, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

isotype

IgMλ

isotype

-

isotype

-

isotype

-

General description

IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory . This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice . IgM isotype control antibody is specific for anti-mouse whole serum and anti-mouse IgM. The product does not react with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3.
Isotype controls are non-reactive immunoglobulins of the same isotype as the primary antibody being used in an application. It is recommended that a non-reactive immunoglobulin of the same isotype and concentration be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.
The purified IgM Isotype Control clone MOPC 104E preparation is non-reactive with antisera to mouse IgA, IgG1, IgG2a, IgG2b, and IgG3. When evaluated in flow cytometry, the IgM Isotype Control did not stain human peripheral blood lymphocytes (PBLs). A FITC Goat anti-Mouse IgM (μ-chain specific), Affinity Isolated Antibody (Product No. F 9259) along with 1 μg of the product was incubated with human PBLs and then evaluated by flow cytometry.

Application

IgM Isotype Control clone MOPC 104E is recommended as a negative control in flow cytometry. Working Concentration: equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry. Equal concentrations of isotype control and primary antibody are recommended for use in flow cytometry.
IgM Isotype Control from murine myeloma has been used:
  • in chromatin immunoprecipitation (ChIP) assay
  • LABScreen assays
  • immunofluorescent staining

IgM isotype control antibodies are suitable for use in immunohistochemistry .

Principle

The specificity of staining by monoclonal antibodies to mouse CD antigens should be verified by establishing the non-specific reagent binding to the target cell population. It is recommended that a concentration and isotype matched mouse myeloma immunoglobulin be included as a negative control for each monoclonal antibody reagent used in the immunoassay procedure.
Sigma Mouse Isotype Controls are supplied to match the concentration of Sigma purified and/or conjugated monoclonal antibodies to CD antigens. DUAL-TAG controls contain 100 μg of each conjugate.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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An opportunistic detection of amoebic gill disease in blue warehou, Seriolella brama Günther, collected from an Atlantic salmon, Salmo salar L., production cage in south eastern Tasmania.
M B Adams et al.
Journal of fish diseases, 31(9), 713-717 (2008-09-13)
Graham Haddock et al.
Microbiology (Reading, England), 156(Pt 10), 3079-3084 (2010-07-10)
Human small and large intestinal tissue was used to study the interaction of Campylobacter jejuni with its target tissue. The strain used for the study was 81-176 (+pVir). Tissue was processed for scanning and transmission electron microscopy, and by immunohistochemistry
Histone H3 lysine 36 tri-methylation is established over the Xist promoter by antisense Tsix transcription and contributes to repressing Xist expression
Ohhata T, et al.
Molecular and cellular biology, MCB-00561 (2015)
HLA antibody identification with single antigen beads compared to conventional methods
El-Awar N, et al.
Human immunology, 66(9), 989-997 (2005)
Histone H3 lysine 36 tri-methylation is established over the Xist promoter by antisense Tsix transcription and contributes to repressing Xist expression
Ohhata T, et al.
Molecular and Cellular Biology, MCB-00561 (2015)

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